FAQ: Possible Problems on Cryosectioning
Instrument related problems
   
01.   What do I do if the specimen does not advance as expected?
   
  1. Check the specimen advance (micrometer) setting to be certain that it is set correctly.
  2. Activate the coarse specimen advance to confirm that the specimen head is advancing.
  3. Select trim setting (e.g. 20µm) and try sec read more ...
02.   How can I remove the specimen disc if it gets stuck or frozen into place?
   
  1. Moisture on the underside of the disc may have caused the disc to freeze to the quick-freeze shelf or specimen head. Apply concentrated alcohol (>70%) around the disc, and carefully remove.
  2. If the disc remains stuck, gentle read more ...
03.   What do I do if there is no display indication and the cryostat doesn’t work
   
  1. Check that the mains lead is connected properly to the outlet and the instrument.
  2. Check to see if the over-current (GFCI) or circuit breaker has been activated. Reset and switch the cryostat on again with the mains power s read more ...
04.   What do I do if the chamber (or object) temperature cannot be controlled?
   
  1. Ensure that there is a minimum clearance distance of 10 cm on all sides of the instrument.
  2. Check that the compressor ventilation grids are not dirty or covered. Remove any obstruction or remove dust with brush or vacuum cle read more ...