What are the key factors necessary for producing high quality frozen sections? FAQ Report Selecting the optimum temperature for the specific tissue being sectioned. Making certain that the cryostat maintains the set temperature for the cabinet and specimen. Trimming the specimen to a size and shape that will optimize sectioning. Eliminating ice crystal artifacts in the specimen by using an appropriate, rapid freezing technique. Attaching the specimen to the specimen holder (chuck) in a manner that will facilitate artifact free sections (minimizing amount and avoiding creating layers of cryocompound). Keeping the cryo-microtome in good working order by keeping it clean, defrosting on a regular basis and lubricating according to manufacturer’s recommendations. Applying the appropriate tension to the clamping system for the knife or blade. Selecting the correct knife angle. Using a sharp, nick-free knife or disposable blade. Properly adjusting the anti-roll plate and making sure it is damage free. Collecting the sections in a manner that preserves the specimens integrity and morphology.