| Slices of very hard materials can be prepared without destroying the morphology of the specimens for the use in light microscopical investigation and section thicknesses of approximately 30 microns can be achieved under optimal conditions. Most objects (such as resin embedded undecalcified bone or teeth) need a thickness of 80-100 µm for best results. The Leica SP1600 is very often used as a pre-preparation instrument for the Leica SM2500 E with Leica SP2600 (Polycut with Ultramiller). With the saw microtome, slices of about 100-500 microns thickness are prepared and finished to a thickness of about 20-30 microns for the investigation with the transmission light microscope using the Leica SP2600. (Limitation of the Leica SP2600: no titanium, steel or ceramic material should be used). The Leica SP1600's operating principle is based on a specimen holder which is guided extremely slowly against the rotating cutting edge of the diamond coated inner-hole saw blade.
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